Help Center1.Is animal component free (ACF) protein available?
All recombinant proteins on sale are Animal Component Free proteins. Don't contain any products directly derived from animals or human.
2.Can you provide natural purified protein?
Currently, Elabscience does not provide natural proteins.
3.Can the protein be used for cell culture/injection of animal?
Elabscience provides recombinant proteins that be used for cell culture or injection of animal.
Generally speaking, proteins with an endotoxin content of less than 0.1 ng/ug (1 EU/ug) can be used in cell culture/injection animal.
4.Can your protein be used for Elisa kit development?
The on sale recombinant proteins can be used to develop Elisa assay. If binding activity is not mentioned in the protein specification, the customer may need to explore the reaction conditions themselves.
5.Can your proteins be used as an Immunogen?
The on sale recombinant proteins can be used as an Immunogen.
6.What solution can be used to dilute recombinant protein?
As lyophilized protein, it is recommended to reconstitute the lyophilized powder to a stock solution refer to hardcopy manual, then dilute with buffer as your experiment needed. As liquid protein, just dilute into target concentration with buffer as your experiment needed.
7.Has the protein been sequenced after it is expressed?
The Recombinant protein was not sequenced directly. But we sequenced the protein expression plasmid, and only if the sequence of target gene is correct can the protein be manufactured.
8.Can you please advise whether any virus or viral vector was used in the expression of the protein that expressed in Eukaryotic expression system?
No virus or viral vector was used in the expression of the protein that expressed in Eukaryotic expression system.
9.How is the protein purified?
Generally, recombinant protein was purified by affinity chromatography with the label, including nickel column/His Tag and protein G/Fc tag. The unlabeled proteins were purified by affinity chromatography first and then removed label.
10.What does "AP Mol Mass" mean? When I calculate molarity, should I consider "Mol Mass" or "AP Mol Mass"?
"AP Mol Mass" means apparent molecular weight, it is measured by SDS-PAGE. "Moll Mass" means theoretical molecular weight, it is calculated by direct translation of coding sequence. It is quite normal that the apparent molecular weight of a protein is not the same with the theoretical molecular weight.
There are complex post-transcriptional modifications of mRNA and post-translational modifications of proteins that result in changes in apparent molecular weight. When calculate molar concentration you should take into account Mol Mass.