Help Center- Target analyte in tested samples
It's recommended to test fresh samples by ELISA, which could avoid false positive or negative results caused by the degradation or decomposition of target analyte during long time storage.
- Dilution factors and diluent
Dilution ratio: refer to the specific protocol or published papers, or consult to our technical support. Email to: techsupport@elabscience.com
Recommended diluent: standard & sample diluent provided in the ELISA kit, we could provide more vials of it for free for specific application, just tell us when you place the order.
- Assay procedure
- Following the protocol strictly, here are some hints
- Establish the complete Standard Curve for each assay
- Ensure the precise volume for each reagent addition
- Try to finish the liquid addition for each reagent as soon as possible, better not over 10 min
- Keep constant speed for liquid addition to each well
To get more accurate experiment result, it’s recommended to conduct the ELISA assay as soon as possible once you receive the kit.
- How to calculate / deal with the raw datas?
After correct the average OD values, plot a four parameter logistic curve on log-log graph paper, with standard concentration on the x-axis and OD values on the y-axis, many softwares could also help to deal with it, such as Origin( recommended), CurveExpert.