Serum: Allow samples to clot for 1 hours at room temperature or overnight at 4℃ before centrifugation for 20 minutes at 1000×g at 2 - 8℃. Collect the supernatant to carry out the assay. Blood collection tubes should be disposable, non-endotoxin.
Plasma: Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples for 15 minutes at 1000×g at 2 - 8℃ within 30 minutes of collection. Collect the supernatant to carry out the assay. Hemolysis samples are not suitable for ELISA assay.

Serum: Allow samples to clot for 1 hours at room temperature or overnight at 4℃ before centrifugation for 20 minutes at 1000×g at 2 - 8℃. Collect the supernatant to carry out the assay. Blood collection tubes should be disposable, non-endotoxin.
Plasma: Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples for 15 minutes at 1000×g at 2 - 8℃ within 30 minutes of collection. Collect the supernatant to carry out the assay. Hemolysis samples are not suitable for ELISA assay.

How to collect serum and plasma for ELISA assay?

ELISA High Background Possible Causes and Solutions

How can I determine whether an antibody may detect in an untested species?

ELISA Poor Standard Curve Possible Causes and Solutions

How to deal with saliva, urine, milk, tear and other samples for ELISA assay?

ELISA Standard Curve Achieved but Poor Discrimination Between Points

ELISA Poor Reproducibility Possible causes and Solutions

ELISA Edge Effect Edge Effect and Solutions

What are the recommended storage conditions for protein?

Questions and Answers of Metabolism Assay Kits

Immunofluorescence Troubleshooting Tips

Immunohistochemistry Troubleshooting Tips

How to deal with tissue or cells samples for ELISA assay?

ELISA Poor Precision Possible Causes and Solutions

Recombinant protein has been shipped in transit for several weeks, and the ice/blue ice packs have melted when package been received, can the protein still be used in experiment?

What are the transport conditions of the protein?

How to storage samples for ELISA assay?

Flow Cytometry Troubleshooting Tips

How to Calculate ELISA Results

ELISA Low signal or No signal

How long can the protein be kept at room temperature?

Western Blot Troubleshooting Tips

How to perform an ELISA assay as perfectly as possible?

Technical matters about the proteins

Troubleshooting for Metabolism Assay Detection

What concentration of primary antibody should I use?

Can the protein be delivered in lyophilized form? Can you deliver liquid form?

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