ELISA Standard Curve Achieved but Poor Discrimination Between Points

1 min. readlast update: 11.25.2021

Problem:Standard curve achieved but poor discrimination between points.

Possible causes 1:Improper calculation of standard curve dilutions

Solutions 1:Check the calculation results and make new standard curve

Possible causes 2:Insufficient washing

Solutions 2:Ensure sufficient washing

Possible causes 3:Plate sealer reused

Solutions 3:Use a fresh plate sealer for each step

Possible causes 4:Not used plate sealers

Solutions 4:Use plate sealers

Possible causes 5:Buffer contaminated

Solutions 5:Prepare fresh buffer

Possible causes 6:Insufficient washing

Solutions 6:Ensure sufficient washing. If using an automatic plate washer, check that all ports are clean and unobstructed.

Possible causes 7:Variations in incubation temperature

Solutions 7:Adhere to recommended incubation temperature; Avoid putting plates in variable environmental conditions.

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