ELISA Poor Standard Curve Possible Causes and Solutions

Problem：Poor standard curve

Possible causes 1：Incomplete washing

Solutions 1：Ensure that the washing apparatus is working correctly.

Possible causes 2：Inadequate aspiration of wells

Solutions 2：Wells should be fully dry after aspiration.

Possible causes 3：Unequal volumes added to wells

Solutions 3：Check pipettes; Recalibrate the pipette if necessary.