ELISA Poor Precision Possible Causes and Solutions

1 min. readlast update: 11.25.2021

Problem: Poor precision

Possible causes 1:Incomplete washing

Solutions 1:Ensure that the washing apparatus is working correctly; Ensure adequate time of soaking.

Possible causes 2:Inadequate aspiration of wells

Solutions 2:Wells should be fully dry after aspiration.

Possible causes 3:Incomplete mixing of reagents

Solutions 3:Ensure adequate mixing.

Possible causes 4:Buffers contaminated

Solutions 4:Prepare fresh buffer.

Possible causes 5:Improper dilution of standard

Solutions 5:Use diluent in the kit as blank value; Ensure accurate dilution of highest standard; Ensure accurate completion of 2-fold dilution series.

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