Problem: Poor precision
Possible causes 1:Incomplete washing
Solutions 1:Ensure that the washing apparatus is working correctly; Ensure adequate time of soaking.
Possible causes 2:Inadequate aspiration of wells
Solutions 2:Wells should be fully dry after aspiration.
Possible causes 3:Incomplete mixing of reagents
Solutions 3:Ensure adequate mixing.
Possible causes 4:Buffers contaminated
Solutions 4:Prepare fresh buffer.
Possible causes 5:Improper dilution of standard
Solutions 5:Use diluent in the kit as blank value; Ensure accurate dilution of highest standard; Ensure accurate completion of 2-fold dilution series.