Problem：Low signal or no signal
<ins>Possible causes 1</ins>：Buffer contaminated
<ins>Solutions 1</ins>：Make fresh buffer.
<ins>Possible causes 2</ins>：Determinant level contained in samples is above the kit’s detection range
<ins>Solutions 2</ins>：Dilute samples and run again
<ins>Possible causes 3</ins>：Incorrect wavelength
<ins>Solutions 3</ins>：Check the filters/reader.
<ins>Possible causes 4</ins>：Stop solution was not added
<ins>Solutions 4</ins>：Add stop solution to each well.
<ins>Possible causes 5</ins>：Insufficient incubation time
<ins>Solutions 5</ins>: Increase the incubation time.
<ins>Possible causes 6</ins>：Incubation temperature vary wildly
<ins>Solutions 6</ins>: Check the incubator.
<ins>Possible causes 7</ins>：Incorrect dilutions of detection Ab/HRP-conjugate
<ins>Solutions 7</ins>: Adhere to the manual.
<ins>Possible causes 8</ins>：The kit has lost its activity
<ins>Solutions 8</ins>: Get a new kit.
<ins>Possible causes 9</ins>：Plate not incubated long enough
<ins>Solutions 9</ins>: Increase Substrate Solution incubation time.

Problem：Low signal or no signal
Possible causes 1：Buffer contaminated
Solutions 1：Make fresh buffer.
Possible causes 2：Determinant level contained in samples is above the kit’s detection range
Solutions 2：Dilute samples and run again
Possible causes 3：Incorrect wavelength
Solutions 3：Check the filters/reader.
Possible causes 4：Stop solution was not added
Solutions 4：Add stop solution to each well.
Possible causes 5：Insufficient incubation time
Solutions 5: Increase the incubation time.
Possible causes 6：Incubation temperature vary wildly
Solutions 6: Check the incubator.
Possible causes 7：Incorrect dilutions of detection Ab/HRP-conjugate
Solutions 7: Adhere to the manual.
Possible causes 8：The kit has lost its activity
Solutions 8: Get a new kit.
Possible causes 9：Plate not incubated long enough
Solutions 9: Increase Substrate Solution incubation time.


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