Problem：High background
<ins>Possible causes 1</ins>：Reagents are not at room temperature
<ins>Solutions 1</ins>：Allow all reagents to reach room temperature (18-25℃) before use.
<ins>Possible causes 2</ins>：Incomplete washing
<ins>Solutions 2</ins>：Ensure that the washing apparatus is working correctly. Ensure adequate time of soaking.
<ins>Possible causes 3</ins>：Inadequate aspiration of wells
<ins>Solutions 3</ins>：Wells should be fully dry after aspiration.

Problem：High background
Possible causes 1：Reagents are not at room temperature
Solutions 1：Allow all reagents to reach room temperature (18-25℃) before use.
Possible causes 2：Incomplete washing
Solutions 2：Ensure that the washing apparatus is working correctly. Ensure adequate time of soaking.
Possible causes 3：Inadequate aspiration of wells
Solutions 3：Wells should be fully dry after aspiration.


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