ELISA Edge Effect Edge Effect and Solutions

Problem：Edge effect

Possible causes 1：Uneven temperatures among wells

Solutions 1：Adhere to recommended incubation time and temperatures.

Possible causes 2：Evaporation caused by inadequate covering of plate sealer

Solutions 2：Ensure correct use of plate sealer.

Possible causes 3：The assay was interrupted

Solutions 3：The assay should be continuous. All standards/samples should be prepared appropriately before the assay.